Z-Stacking and Z-Projection using a Scaffold-based 3D Cell Culture Model

Z-projection is a digital image processing method, which combines multiple images taken at different focal distances to provide a composite image with a greater depth of field than any of the individual source images. This application note, demonstrates this technique using Gen5™ Image+ Data Analysis Software to perform z-stacking and z-projection of scaffold-based 3D cellular tumoroid structure images.