ZEISS Lightsheet Z.1 Quick Guide: Imaging with Water or Clearing Immersion

The ZEISS Lightsheet Z.1 imaging system is capable of delivering optical sections of large samples, with virtually no phototoxicity or bleaching and with high temporal resolution. Light Sheet Fluorescence Microscopy has advantages for imaging live as well as cleared samples, with the Lightsheet Z.1 as equally capable in clearing applications. This document describes the necessary steps to change between water immersion and clearing immersion imaging options.