Search results for "kings college london"

A simple and straightfor­ward procedure for measuring functional kinase activity.The Eu(K) labeled antibody and XL665 conjugate are added simul­taneously after the enzymatic step. Results are available after a 2-hour incubation at room temperature. The two reagents are stable in solution, even in an excess of EDTA (0.5M), while the assay remains stable for >24 hours. An extended list of Eu3+-Cryptate labeled phos­pho-specific…

CRISPR/CAS9 Gene Editing of Human Induced Pluripotent Stem Cells (iPSCs)  

Streamline gene activation experiments with stably expressing dCas9-VPR cell lines

CompoZr ® ZFN (zinc finger nuclease) technology was used to insert the sequence for fluorescent proteins adjacent to endogenous genes to create reporter cell lines for compound screening

This pooled library targets the human kinome, a collection of approximately 700 genes commonly associated with development and disease, with an average of nearly 9 unique knockout CRISPR clones per kinase gene. The lentiviral format allows for stable integration, selection, and enrichment of transduced cells

Components of the Upstate (Millipore) Casein Kinase 2 Assay Kit:• Casein Kinase 2 Substrate Peptide (Cat.# 12-330) • Assay Dilution Buffer I (ADBI) (Cat.# 20-108) • PKA Inhibitor Cocktail (Cat.# 20-114) • Magnesium/ATP Cocktail (Cat.# 20-113) • P81 Phosphocellulose Squares (Cat.# 20-134) For use to measure the phosphotransferase activity of Casein Kinase 2 (CK-2) in immunoprecipitates and column fractions based on phosphorylat…

Corning® Collagen I, rat tail, 1g (10 x 100mg), is used either as a thin layer on tissue-culture surfaces to enhance cell attachment and proliferation, or as a gel to promote expression of cell-specific morphology and function. Source Rat Tail Tendon Quality Purity = 90% by SDS-PAGE Tested for ability to promote attachment and spreading of HT-1080 human fibrosarcoma cells Tested for formation of a firm gel up to a dilu…