Absolute Quantification of Nucleic Acids with the Droplet Digital™ PCR System

In this SelectScience.tv interview at AACR 2011, QuantaLife introduce the Droplet Digital™ PCR system. The Droplet Generator partitions each sample into twenty thousand one nanolitre droplets. A PCR reaction runs to completion in each droplet, and every droplet of sample is read by the Droplet Reader as either positive or negative for the target DNA/RNA molecules. Statistics are used to estimate the concentration of the target in the original sample, giving digital absolute quantification.