Automated Two-Step Purification of Mouse Antibody IgG1

Dr. Yannick Krauke from the KNAUER lab describes an automated purification of mouse immunoglobulin antibodies (IgG) from cell culture. The cell culture was applied with a feeding pump on a proteinA affinity column to capture and purify the antibodies. These were kept in the system and in a second step applied on a gel filtration column for buffer exchange. The obtained antibody was now dissolved in the desired storage and/or working buffer. This application note highlights the possibility of automated purification with the AZURA BIO LC system without manual interaction during purification process.