Cell line engineering with CRISPR-Cas9: Guide RNA & donors for knock-in

Generating a knock-in cell line adds another line of complexity to the relatively straightforward knockout application. Guide RNA can be more tricky to design as you not only need more efficient guides, but they also need to be in close proximity to your insert site of interest. John Schiel, Ph.D., knock-in expert at Horizon Discovery shares how Horizon's tools for site specific editing and donor design aid in generating the sequences for these reagents as well as what donor formats make sense based on your type of insert.