Cellular Dynamics: Capillary-like Network Formation of iCell® Endothelial Cells in PEG Hydrogel

These time-lapse video images illustrate 3D capillary-like network formation for iCell® Endothelial Cells (Cellular Dynamics International) encapsulated in a synthetic polyethylene glycol (PEG) hydrogel. Images shown for time points recorded over days 2-3 in culture. The PEG hydrogel was formed with protease-degradable crosslinks and a pendant adhesion peptide to promote cellular remodeling, and grown in standard CDI-recommended media & supplement. Video courtesy of Michael Schwartz, University of Wisconsin.