Combining orthogonal glycan analysis techniques for a deeper understanding of a tri-specific protein

Explore the critical role of glycosylation in multispecific proteins and its impact on their function. Michael McKinnon, from Novartis Institutes for Biomedical Research, highlights traditional glycosylation analysis methods and presents a case study on a TriSpecific fusion protein, emphasizing the need for precise glycan localization. The discussion compares Electron-Activated Dissociation (EAD) and Collision-Induced Dissociation (CID) techniques, showcasing their applications in glycan analysis. The ZenoTof 7600 is introduced as a powerful tool for advanced protein characterization, providing deeper insights into glycan structures and modifications.