Live Cell Imaging of Apoptosis in Immune Cells using the CellASIC ONIX2 Microfluidic System

Jurkat cells were first loaded onto the CellASIC^® ONIX2 System M04T Pad Trap Plate and were then incubated with BioTracker NucView^® 488 Green Caspase-3 Dye and Annexin V Texas Red for the first 2 hours in complete RPMI-1640 media, followed by treatment of 2.5 µg/mL CD-95 antibody or no treatment for 8 hours. Time-lapse imaging was taken on cells at a 10-minute interval under 400x magnification using a FITC and Texas-Red filter.