Processing forensic samples using a single-tube approach

Reducing agents, such as DTT, have been used to extract DNA from sperm cells in forensic sexual assault samples for many years. These reducing agents require harsh detergents and denaturants in the lysis buffer to reduce disulfide bonds. The resulting lysate contains reagents and proteins that must be removed via purification steps prior to qPCR and/or STR amplification. In this webinar, Jeff Hickey from MicroGEM, demonstrates how a single-tube protocol can help efficiently process sexual assault samples, including Y screening and differential extractions.