Real-time Monitoring of Phagocytosis Using the IncuCyte Instrument

Real-time monitoring of phagocytosis using the IncuCyte^® live-cell imaging & analysis system. Time-lapse phase contrast and fluorescence images, acquired using IncuCyte instrument, enable the real-time visualisation of IncuCyte™ pHrodo^® Green E. coli Bioparticles^® engulfment by the mouse macrophage cell type J774A.1. The Bioparticles are phagocytosed and enter the acidic environment of the phagosome resulting in increased fluorescence of the pHrodo^® label. IncuCyte integrated image analysis tools enable detection and measurement of the green fluorescent signal over the entire assay time-course and eliminate the impact of background fluorescence.