Real-time Monitoring of the Phagocytosis using IncuCyte™ pHrodo Green E. coli Bioparticles

This video shows real-time monitoring of the phagocytosis of IncuCyte™ pHrodo^® Green E. coli Bioparticles^® using the IncuCyte ZOOM^® live-cell imaging system. Time-lapse fluorescence images, acquired using IncuCyte ZOOM^®, enable the real-time visualisation of IncuCyte^® pHrodo^® Green E. coli Bioparticles^® engulfment by the mouse macrophage cell type J774A.1. The Bioparticles^® are phagocytosed and enter the acidic environment of the phagosome resulting in increased fluorescence of the pHrodo^® label. IncuCyte^® ZOOM integrated image analysis tools enable detection and measurement of the green fluorescent signal over the entire assay time-course (more than 24 hrs) and eliminate the impact of background fluorescence.