What is Microfluidic Diffusional Sizing?

Microfluidic Diffusional Sizing (MDS) is a novel solution-phase technology for measuring binding affinity and characterizing protein interactions.

Unlike traditional methods such as surface plasmon resonance (SPR) and bio-layer interferometry (BLI), MDS does not rely on surface immobilization or purification, making it possible to easily work with a wide variety of targets across all sample types — from small peptides to large protein complexes, and in simple buffers to complex biofluids like plasma or serum.

Explore how MDS provides a predictable and direct measurement: the molecular size, or hydrodynamic radius (R_h), which allows scientists to determine binding affinity and calibration-free concentration or binding stoichiometry — all at once.