3D Tumor Modeling: Latest Solutions for Single and Multi-Spheroid Culture & Analysis

In the search for greater accuracy in vitro, researchers are increasingly turning to more complex live-cell assay systems that recapitulate key aspects of in vivo conditions. These include (1) more relevant tissue organization, (2) bio-matrices containing structural proteins and growth factors, and (3) the presence of stromal and immune cells. In this event, we describe integrated protocols and live-cell analysis solutions for different 3D tumor models, ranging from simple mono-culture single spheroid assays through to multi-spheroid tumor/fibroblast and tumor/immune cell co-cultures in a Matrigel biomatrix.