Achieve successful sequencing data based on input sample and library quality control

Next-generation sequencing (NGS) has transformed the way genomes are analyzed. As technologies trend towards higher throughput, lower cost, and improved data quality, standardization and quality control remain key topics in the NGS workflow.

In this SelectScience NGS webinar series, produced in partnership with Agilent Technologies, we explore the importance of input sample quality control (QC). The webinar demonstrates how sample QC relates to the quality of downstream sequencing results and examines key QC aspects to consider when scaling NGS throughput and automation. This series features presentations from expert speakers with a variety of scientific backgrounds and consists of two webinars, starting on Friday, 6 May 2022.

Webinar 1: Achieve successful sequencing data based on input sample and library quality control

This webinar will demonstrate how rigorous quality control (QC) of samples and libraries is crucial to producing high-quality data in next-generation sequencing (NGS). Sample QC metrics like concentration, DNA and RNA integrity numbers, 28S/18S ratio, DV200, fragment length, and amount of adapter dimers will be covered. How these metrics provide reasonable estimates for downstream NGS sequencing parameters such as on-target rate, duplication rate, and sequencing coverage will be discussed.

Carsten Maus, Ph.D., is a bioinformatician at the NGS Core Facility of the German Cancer Research Center (DKFZ) in Heidelberg. Dr. Maus shares his experience on the dependencies between the quality of samples, libraries, and data within a high-throughput sequencing unit.

Rainer Nitsche, Ph.D., Product Manager, Agilent Technologies, will give a brief overview of the Agilent TapeStation systems and how they can be used to collect QC metrics from RNA and DNA samples.

Key learning objectives

• The connection between key NGS QC metrics and downstream sequencing results
• Methods to secure optimal NGS results by applying sample QC
• Advantages of the TapeStation automated electrophoresis systems

Who should attend?

• Genomics researchers using NGS
• Molecular biologists
• Cancer researchers
• Lab managers and technicians

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.