Advancing light scattering assays for AAV products
March 19, 15:00 GMT/ 16:00 CET/ 11:00 EDT/ 08:00 PDT
AAV-based gene therapy products are heterogeneous, composed of empty, partial, and full monomer capsid species as well as capsid aggregates and fragments. Join our exclusive webinar where Dr Margaret Butko, Director of CMC Assay Development, Adverum and Dr. Prithwijit Sarkar, Sr. System Account Manager, Waters|Wyatt Technology, will present two methods: GMP compliant DLS which is used for monitoring capsid aggregation, including generation of a capsid aggregation standard, and the FFF-MALS method, used for quantifying the relative abundance of capsid aggregates.
Explore these two methods, with the addition of quantification platform SEC-MALS, to quantify the relative abundance of both capsid aggregates and full capsids. Find out how you can elevate your AAV-based gene therapy research with these tools and ensure the delivery of consistent products to patients.
Key learning objectives:
- Explore DLS applications to monitor capsid aggregation in a GMP setting
- Discover FFF-MALS applications to quantify the relative abundance of capsid aggregates
- Investigate SEC-MALS applications to quantify the relative abundance of full capsids
Who should attend?
- CMC teams supporting AAV-based therapies
- Scientists involved in AAV characterization, analytical method development, process development, and quality control
- Lab managers that need to select and support an optimal set of AAV characterization methods
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
Speakers
Dr. Margaret Butko is Director of CMC Assay Development at Adverum, leading protein analytical and biophysical characterization. Dr. Butko has 12 years of experience in analytical and biophysical characterization supporting small molecule discovery, therapeutic antibody development, and gene therapy development. She earned her B.A. in chemistry from the College of Wooster and her Ph.D. in biomedical sciences from the University of California, San Diego. Following this she then completed a postdoctoral fellowship at Genentech in the protein analytical chemistry department.
Dr. Prithwijit Sarkar is a Senior System Account Manager at Wyatt Technology and is an expert in applying light scattering techniques to the characterization of a wide variety of biological macromolecules. He graduated from the University of Texas at Dallas, with a Ph.D. in Cellular and Molecular biology. Dr. Sarkar then joined Wyatt Technology in 2020 as a Field Application Scientist, before transitioning into his current position as an Account Manager in 2022.
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