Analysis of the effect of base-pair mismatch on DNA melting temperatures through UV-visible absorption spectroscopy

Double-stranded DNA can play an important role in a variety of pharmaceutical formulation procedures, including vaccine development. The melting temperature, or temperature at which the double-stranded DNA disassociates into its single-stranded parts, is characteristic of the specific sequence used and can be determined through temperature-dependent UV-visible absorption measurements.

The observed melting temperature of a DNA sequence can be sensitive to changes in the buffer concentration, species present in solution, and the presence of base-pair mismatches within the sequence. As a result, the determination of a specific sequence’s melting temperature can be used as a diagnostic for changes in the anticipated sequence makeup or solvent environment.

In this webinar, we demonstrate the ability to use UV-visible absorption spectroscopy to determine the melting temperature of double-stranded DNA in different environments, including sequences containing base-pair mismatches.

Key learning objectives

• Learn how to use UV-visible absorption techniques to determine the melting temperature of double-stranded DNA
• Understand the environmental factors which can change the calculated melting temperature of a DNA sample

Who should attend?

Scientists working in biopharma or pharma working with DNA.

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.