Automated NGS library preparation for oncology application using the Biomek NGeniuS System
Preparing next-generation sequencing (NGS) libraries manually can be labor-intensive and time-consuming, taking anywhere from a few hours to several days depending on the type of library being created.
Join Dr. Jeffrey Wilson and Zach Smith, Staff Application Scientists at Beckman Coulter Life Sciences, as they demonstrate the automation of two distinct NGS library preparation kits using the Biomek NGeniuS System utilized in oncology research.
The Pillar Biosciences oncoReveal Solid Tumor 22 Gene Panel Kit utilizes proprietary amplification technology and a small panel targeting 22 genes to amplify the target regions. The Illumina TruSight Oncology 500 DNA/RNA kit uses an alternative strategy involving NGS library preparation followed by hybridization/capture using a much larger panel of 523 genes along with other markers to develop a comprehensive genomic profile of the sample.
Key learning objectives:
- Gain insights into the features and automation capabilities of the Biomek NGeniuS system, including its role in laboratory efficiency
- Explore the Pillar and TSO 500 library preparation workflows, including the specific cancer variants and mutants they target for enrichment and amplification
- Learn how the Biomek NGeniuS system can manage two distinct workflows, highlighting its flexibility and adaptability in different research settings
- Examine the data generated from automating the Pillar and TSO workflows on the Biomek NGeniuS, and the implications for cancer testing laboratories, including improvements in accuracy and efficiency
Who should attend?
- Professionals involved in cancer research, including researcher, academics and clinical researchers.
- Those interested in NGS and automation.
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
Speakers
Dr. Jeffrey Wilson has worked at Beckman Coulter Life Sciences for over 9 years. He was involved in the Biomek NGeniuS Next Generation Library Preparation System software development and currently supports its application development. He has been working with NGS chemistry vendor partners to advise and train them on the Biomek NGeniuS platform, and supports translating the NGS chemistries accurately on the automated system. He has a Ph.D. in Chemistry from the University of Arkansas and was a postdoctoral fellow at the University of Cincinnati College of Medicine and Cincinnati Children's Research Foundation.
Zach Smith has worked at Beckman Coulter Life Sciences for over 10 years. He has supported next-generation sequencing (NGS) and genomics applications on a wide range of Biomek liquid handling platforms. Most recently at Beckman Coulter, he was actively involved in the development of the Biomek NGeniuS Next Generation Library Preparation System and now supports applications development on that platform as well as the Beckman Coulter Biomek iSeries liquid handlers. He has a BSc in molecular biology and a master’s in environmental toxicology, both from Indiana University.
Moderator
