Automated spheroid production by magnetic cell assembly for reproducible HTS applications

Cells grown in a 3D environment more accurately recapitulate the in vivo situation of native tissues. 3D models, therefore, show a higher physiological relevance in terms of proliferation, differentiation, metabolism, and gene expression. Many 3D cell culture methods have been developed with great potential for use as more realistic models in tissue engineering, drug development, and basic research.

One approach to generate 3D cell cultures is to use in vitro aggregation of cells to produce spheroids, and this has become an essential tool in research today. However, there are currently challenges in spheroid production related to uniformity, variations in size, and compatibility with automation for high-throughput screening applications. In addition, tissue thickness and plate geometry can impair the quality of imaging-based readouts.

In this webinar, we will outline an automated workflow to tackle these limitations using magnetic 3D cell culture (M3D) for the rapid generation of spheroids in a highly reproducible manner.

Key learning objectives

• Learn how to utilize magnetic 3D bioprinting to enable automation and optimal imaging conditions
• Discover how to automate spheroid production to reduce uniformity variations
• Consider how to automatically monitor QC for spheroid formation and production
• Find out how to simplify and automate drug dosing for synergy experiments

Who should attend?

• Academic scientists interested in the techniques and applications of 3D cell models for research, engineering, and drug development
• Industry scientists interested in identifying promising platforms for drug discovery and targets for novel therapies

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

Tecan D300e Digital Dispenser and Spark Cyto are for research use only. Not for use in diagnostic procedures. Tecan D300e Digital Dispenser is a product of HP Inc. CA, USA.