Characterizing critical quality attributes of outer membrane vesicles for conjugate vaccines

With a size comparable to that of viruses and self-adjuvating properties, outer membrane vesicles (OMVs) represent an excellent alternative to traditional carrier proteins for conjugate vaccines. An essential aspect of developing OMVs as vaccine products is characterization with respect to size and purity. Asymmetric-flow field-flow fractionation coupled with multi-angle light scattering (AF4-MALS) is an invaluable technique for bionanoparticle analysis, with an AF4-MALS method for quantifying OMV critical quality attributes recently being published in Analytical Chemistry.

This webinar will introduce AF4-MALS and OMV, and review the validation program that was established to assess the quality attributes obtained by AF4-MALS according to the principles described in ICH Guidelines Q2 (R1).

Key learning objectives

• Understand the principles and technology of AF4-MALS
• Discover the strategies and parameters for AF4-MALS method development
• Learn how to assess OMV quality attributes by AF4-MALS
• Consider AF4-MALS validation using ICH guidelines

Who should attend?

• Scientists developing analytical methods for bionanoparticles and vaccines
• Vaccine development scientists in need of robust, reliable, methods to characterize size and purity

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.