CRISPR without the cut: a novel CRISPR interference system enabling rapid functional gene characterization

This webinar will highlight the Dharmacon™ CRISPRmod CRISPRi system as detailed in a peer-reviewed article in The CRISPR Journal.

Learn first-hand from the inventor about its proprietary effector specifically developed for use with synthetic single guide RNAs (sgRNAs). The system enables functional gene characterization in arrayed format experiments making it a powerful, orthogonal method to siRNA and CRISPR knockout screening.

Key learning objectives

• Learn about a novel CRISPRi effector, dCas9-SALL1-SDS3, that mediates more potent repression when used with synthetic sgRNAs than previously described effectors such as dCas9-KRAB and dCas9-KRAB-MeCP2.
• Discover how dCas9-SALL1-SDS3 interacts with key members of the histone deacetylase and Swi-independent 3 complexes to mediate rapid, potent target gene repression.
• Learn about the development of in vitro-transcribed dCas9-SALL1-SDS3 mRNA for delivery into clinically relevant cell types such as human induced pluripotent stem cells and primary T cells.
• Explore the functional gene characterization of DNA damage host factors using dCas9-SALL1-SDS3 with synthetic sgRNAs, demonstrating the ability of the system to be used in arrayed-format screening.

Who should attend?

• Academics and industry professionals interested in functional genomics, specifically gene editing and gene modulation (CRISPR-Cas9 and RNAi technologies)

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.