Examining T-cell dynamics in the human brain and its borders using cell sorting, genomics and inter-cell communication mapping tools

The Elyaman lab at Columbia University studies the recruitment and role of adaptive T cells in neurodegenerative diseases including Alzheimer's disease and Parkinson's disease. Capitalizing on advancements in microfluidics chip-based cell sorting capabilities of the MA900 Multi-Application Cell Sorter and single cell sequencing technologies, this lab has delineated a first-in-human comprehensive view of the immunological landscape in the brain and its borders. The ultimate goal of these studies is to better understand how the brain-border immunity is altered in human neurodegeneration and its effects on CNS inflammation.

In line with previous mouse work, the lab has found that resident T cells in the choroid plexus likely play an immunoregulatory role that controls migration of immune cells across the blood/CSF barrier.

Join this webinar to:

• Learn about the approaches used for processing fresh postmortem leptomeninges, choroid plexus, and brain from patients across multiple neurodegenerative diseases and for isolating highly pure and viable immune cells using the MA900 Cell Sorter from Sony Biotechnology.
• Understand how computational tools like CellChat can be used to explore the information about shared TCRs and paired RNA between the meninges and brain to infer intercellular interactions controlling T-cell activity, recruitment, and activation.
• Learn how T cells undergo clonal expansion at the brain’s borders and form separate reservoirs in the meninges, brain, and the choroid plexus.

Who should attend?

• This webinar will provide insights to researchers who want to learn about the strategies for developing a robust and gentle immune cell enrichment pipeline and learn about the involvement of adaptive immunity in neurodegenerative diseases.

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.