Exploring single-cell biophysics with a simple, robust microfluidics system
Live-cell analysis monitors rapidly changing living processes. Scientists recognize the need for observing cellular changes over extended periods of time to truly understand biological systems. A major limitation with traditional static cell culture methodologies is the lack of a highly controlled, in vivo-like dynamic environment.
In this exclusive webinar, we will review the power of microfluidics for dynamic time-lapse experiments. Beyond this, a perfusion-based control system integrating imaging capabilities will be introduced, which permits optimal cell health over long time periods, and provides the ability to precisely manipulate environmental parameters and measure cellular responses in real time.
Dr. Enrique Rojas describes his application subjecting bacteria to rapid oscillatory osmotic shocks for studying the biomechanical basis of cell growth and division. In gram-negative strains, Rojas demonstrates the outer membrane’s significance for sustaining mechanical perturbations and growing in the presence of antibiotics. Additionally, Rojas discusses the ecology of bacteria-bacteriophage interactions and the genetic basis of cell growth.
Key learning objectives:
The use of microfluidics for time-lapse microscopy. How to monitor cells (single-cell level) in real time, while delivering precise and rapid changes to culture parameters, including temperature, media and gas. Understand perfusion cell culture systems that provide optimal representation of natural physiological conditions. How microfluidic systems integrated with inverted microscopy allow continuous, high magnification observation of live cells, while capturing their reactions to environmental perturbations in real time.
Who should attend?
- Researchers who use live-cell imaging.
- Researchers who study host-pathogen interactions, protein translocation, hypoxia, cell migration, autophagy, 3D cell culture and primary neuronal cultures.
- Researchers conducting time-lapse microscopy.
- Researchers utilizing bacterial, mammalian or yeast systems.