Exploring topics in sample QC for NGS workflows: Solutions for special challenges in NGS workflows
During next-generation sequencing (NGS) library preparation, examining DNA sample molarity may provide better visualization for long-read sequencing over traditional sample mass measurements. Part one of the webinar will discuss how the Agilent Femto Pulse system and Agilent ProSize data analysis software can be used to determine sample molarity to predict long-read sequencing read lengths.
The presence of adapter dimers in a NGS library can have detrimental effects on downstream sequencing results, such as reduced output, lower diversity, and decreased genome coverage. Part two of this webinar highlights the sensitivity of the Agilent automated electrophoresis portfolio to detect adapter dimers and provide valuable library quality data.
Key learning objectives
- Learn how to examine sample molarity for improved visual representation of sequencing read number
- Understand how to use data visualization in moles and smear analysis to conduct informed size selection experiments prior to long-read sequencing
- Discover why detection of adapter dimers in NGS libraries is needed during NGS library quality control stages
- Learn how to obtain valuable NGS library quality data using Agilent automated electrophoresis instruments
Who should attend?
- NGS and genomics researchers
- Molecular biologists
- Cancer researchers
- Sequencing lab managers and technicians
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.