HPTLC Online User Meeting, hosted by the HPTLC Association
Promoting the use of HPTLC in plant analysis and other analytical fields is the main purpose of the International Association for the Advancement of High-Performance Thin-Layer Chromatography (HPTLC Association), and for this, bringing together representatives from academia, industry, research, regulatory and standard setting bodies.
This HPTLC Online User Meeting is the first in a series of quarterly events that shall facilitate a worldwide exchange of knowledge between HPTLC users to share in the spirit of working in an exciting analytical field.
The meeting is hosted in partnership with the North America Chapter of the HPTLC Association.
Session 1 – Identification of Elderberry (S. nigra) using HPTLC, HPLC and UV-Vis and Detection of its Adulterants in the World of Dietary Supplements
The European elder (Sambucus nigra) berry has a long-standing use in herbal medicine. With COVID-19 on the rise in 2020, the demand for a natural boost to the immune system brought elderberry to the forefront of dietary supplements and herbal medicines. This increasing demand also saw an increase in adulteration. In this session, Brittany Brodziski, QC Supervisor at Nature’s Way, will describe how her team used an orthogonal approach to confirm the quality of the sourced ingredients and for testing raw materials by HPTLC, HPLC, and UV-Vis. Brodziski will further discuss how competitor products were also tested, with a significant number showing low quality or adulteration.
Session 2 – HPTLC Separation of Tryptamine-based Hallucinogens
TLC has often been used for the analysis of common hallucinogenic tryptamines. However, TLC does not always provide a satisfactory level of sensitivity, resolution, or documentation. In this session, Kelsey Patterson, graduate student at Cedar Crest College, discusses her project evaluating the suitability of HPTLC to determine an optimal separation of 12 hallucinogenic tryptamines. Patterson will discuss how several different mobile phases were investigated and explain how two of the mobile phases tested, when used in conjunction with p-dimethylaminobenzaldehyde derivatization, were able to separate and distinguish all 12 tryptamine compounds.
Session 3 – HPTLC/MS Analysis and screening of Banisteriopsis caapi and Psychotria viridis for the purpose of determining and minimizing variability of the active compounds in the final product, with the intention of predicting and improving the quality and potential efficacy of the Amazonian therapeutic botanical medicine admixture, Ayahuasca
HPTLC/MS analysis and screening of active compounds in Ayahuasca traditional medicine has been carried out with the intention of optimizing its therapeutic variability. In the final session of this event, Dr. Sidney Sudberg, President & CSO at Alkemist Labs, will discuss how the relative concentrations of known active compounds were determined by image evaluation, scanning densitometry, and HPLC, and explore how mass spectrometry was used for accurate substance identification. Additionally, Sudberg will demonstrate how the alkaloid fraction was utilized for determination of identity and for determination of lot-to-lot consistency of raw materials.
Meet the speakers and representatives from the HPTLC Association
Take the opportunity to engage in discussions with speakers, colleagues, and representatives from the HPTLC Association in a moderated Zoom meeting, scheduled immediately after the main event. A separate registration for the Zoom meeting is not required – an access link will be sent to all registrants of the main event.
Key learning objectives
- Expand your knowledge of HPTLC and understand the benefits of HPTLC for diverse applications
- Get insights into the daily work of HPTLC users
- Learn how to optimize your HPTLC application(s)
Who should attend?
- Researchers in analytical science
- Students interested in analytical/plant science
- Analysts
- Technicians
- Members of the HPTLC Association