Improving the sensitivity of LC-MS methods for characterization and quantitation of synthetic oligonucleotides
The primary method for quantitation and characterization of synthetic oligonucleotides is liquid chromatography-mass spectrometry (LC-MS). Whether for characterizing process and product-related impurities or bioanalytical pharmacokinetic studies, improving method sensitivity is of critical importance.
In this presentation, Brian Rivera from Phenomenex will discuss how LC-MS sensitivity can be increased by mobile phase optimization and column selection. He will also demonstrate how to minimize adduct formation and enhance ionization efficiency, which can in turn increase MS sensitivity for improved characterization and quantitation data.
Key learning objectives:
- Learn the fundamentals of electrospray ionization of oligonucleotides, and how different method parameters can affect performance
- Understand the role of mobile phase and column chemistry in LC-MS methods for oligonucleotides
- Determine experimental parameters to ensure high-sensitivity, robust LC-MS methods for synthetic oligonucleotides
Who should attend?
- Analytical method developers looking to improve separation and method robustness for oligonucleotides
- Bioanalytical scientists needing to improve method sensitivity
- Anyone new to oligonucleotide characterization by LC-MS
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.