Innovative sgRNA chromatography method for in vivo and ex vivo CRISPR/Cas genome editing

Wednesday, June 25, 2025 at 16:00 BST / 17:00 CEST / 11:00 EDT / 8:00 PDT

Discover an innovative method for purifying CRISPR single guide RNAs (sgRNAs). This GMP-validated technique ensures over 80% full-length purity, as verified by high-resolution HPLC methods, and complies with the latest FDA standards, which require at least 80% purity for full-length sgRNA products.

Join our expert webinar to learn how Agilent meets these requirements through an orthogonal chromatography approach that employs dual separation modes. This method, which utilizes Agilent’s PLRP-S purification resin alongside strong anion exchange purification, consistently achieves over 80% purity for gRNAs longer than 100 nucleotides, and is actively used in GMP production.

During the webinar, you will learn about the FDA CBER guidance on CRISPR sgRNA purity, how to use analytical chromatography for quality comparison, and Agilent’s optimized oligonucleotide synthesis and orthogonal chromatography approach.

Key learning objectives

• Explore updated FDA CBER guidelines for CRISPR sgRNA purity and impurity detection levels.
• Learn about comprehensive analytics for sgRNA identity, purity/impurity analysis.
• Understand how combining strong anion exchange and reverse phase chromatography in orthogonal purification overcomes the limitations of single methods and achieves higher purity levels.
• Discover how high-resolution HPLC methods assess CRISPR sgRNA purity and ensure analytical comparability across various research and production stages.
• Gain insights into developing and qualifying dual purification processes, including using medium pressure equipment and optimizing buffer systems for high purity and step recovery.

Who should attend?

• External manufacturing, CMC, and supply chain managers: Managers overseeing CDMO partnerships and ensuring regulatory compliance.
• Analytical development and chemistry directors: Experts in transferring, developing, qualifying, and validating analytical methods for nucleic acid therapeutics.
• Technical leads and program managers: Leaders in developing and optimizing CRISPR technologies, gene editing, and RNA therapeutics.
• Quality assurance and quality control specialists: Professionals ensuring FDA compliance and maintaining high-purity standards in sgRNA production.

Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.