Method Development Strategies for Purity of Proteins by Intact and Subunit Analysis

Join this webinar to discover the importance of particle morphology and how it affects method development, specifically for large molecules such as intact monoclonal antibodies (mAbs). The webinar will also include proposed best practices in method optimization, including how to best adjust method parameters such as temperature, gradient slope, and flow rate to obtain high-quality impurity profiling and ensure method robustness and reproducibility.

Key learning objectives

• Explore method development on a wide pore C4 column chemistry to achieve faster run times and optimal separation
• Learn how to optimize temperature, flow rate, and gradients for intact mAb analysis
• Understand how to achieve better reproducibility and resolution of intact mAb and sub-unit analysis

Who should attend?

• Scientists interested in developing a robust, transferable method for proteins
• Scientists looking to improve their intact and subunit protein analysis
• Scientists who want to learn more about column selection and improve column lifetimes

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purpose.