Overcome Oligonucleotide Analysis Challenges with 2D-LC/MS
With the enormous potential of oligonucleotide therapeutics, there is more interest than ever in choosing the best tools for oligonucleotide synthesis, analysis, and purification. Oligonucleotides synthesized using phosphoramidite chemistry are typically analyzed and purified using ion-pair reversed-phase liquid chromatography (IP-RPLC) and anion-exchange chromatography. Since highly salty mobile phases are not compatible with mass spectrometry, and samples need to be desalted to participate in ion-pairing prior to IP-RPLC analysis, investigators can either manually prepare samples or consider using 2D-LC/MS to obtain a mass measurement from salty first dimension separations. 2D-LC increases the workflow speed and avoids manual sample preparation. Given the complex impurity profiles, varying sequence modifications, and lengths, thoughtful optimization of HPLC methodologies for characterization and purification is essential to obtain pure oligonucleotides for research, diagnostics, and therapeutics. This webinar will discuss the common challenges associated with oligonucleotide analysis and purification and how to overcome them through 2D-LC/MS and method optimization.
Key learning objectives
- Understand how to couple multiple LC methods for oligonucleotide detection into one seamless application
- Simplify transitions from 1D salty buffers to a 2nd dimension LC-MS separation
- Technical challenges and impurities associated with oligonucleotide synthesis
- Oligonucleotide analysis with varying oligonucleotide structures (size, modifications)
- Method optimization considerations for ion pair reverse-phase purification and for MS analysis
Who should attend?
- Analytical lab managers
- Lab directors
- Analysts
- Oligonucleotide synthesists
- Academic researchers
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.