Real-time visualization and quantification of Akt activity using live-cell imaging

Kinase signaling plays a key role in coupling extracellular stimuli with numerous downstream cellular functions including proliferation, survival, and migration. These pathways are highly interconnected, and their dysregulation has been implicated in several disease processes, including cancer initiation and metastasis, and chronic inflammation. Akt is a serine/threonine protein kinase that is upregulated across various diseases and has been extensively studied as a therapeutic target. Studying dynamic changes in kinase activity can be difficult, with standard approaches being limited to endpoint assays which cannot monitor the effects of treatment over time.

In this webinar, John Rauch, senior scientist at Sartorius, and Jasmine Trigg, scientist at Sartorius, will demonstrate the utility of the Incucyte^® Kinase Akt Lentivirus Reagent, encoding a kinase translocation reporter based on a green fluorescent protein-tagged Akt substrate whose subcellular localization is phosphorylation-dependent, and a red fluorescent nuclear protein to denote the nuclear/cytoplasmic boundary. This biosensor enables Akt activity to be monitored in real-time, providing kinetic data on Akt activation and inhibition in living cells within a physiologically relevant environment.

Key learning objectives

Gain an overview of validated assays combining the Incucyte^® Kinase Akt Lentivirus Reagent and the Incucyte^® Live-Cell Analysis System for image-based fluorescent readouts of Akt activity.
Discover guidance on experimental set-up and how live-cell analysis can be built into your development workflow.
Learn about case study data to support the use of live-cell analysis within the fields of oncology, inflammation and neuroscience.

Who should attend?

Researchers involved in fundamental research and therapeutic development.
Scientists interested in quantifying Akt activity, and its modulation, within the fields of oncology, inflammation, and neuroscience.
Individuals who desire to implement live-cell imaging into their workflow.

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.