Scaling drug response experiments in liver spheroids: Multiplexing 3D cell-based assays and imaging in a microwell platform
To meet the imperative of minimizing animal testing and reducing drug attrition, advanced in vitro models like 3D cell cultures have gained prominence. Liver 3D spheroids, an early application of these models, have improved drug candidate safety evaluations, however, challenges remain, including reproducibility and automation.
In this webinar, join our experts as they discuss the Gri3D®, a novel hydrogel microwell 96-well plate that facilitates uniform cell seeding, microtissue formation in suspension-like conditions, and automated cell imaging. Learn more about a comparative study, where hepatocyte spheroids were grown in Gri3D® microwells and in ultra-low-attachment (ULA) U-bottom 96-well plates simultaneously. Drug effects on viability and enzymatic activities were assessed using Promega assays (CellTiterGlo®-3D, CellToxTM Green and P450-GloTM.) and Tecan’s Spark® Cyto multi-mode imaging plate reader. Our speakers will explore how the innovative 3D multiplexing approach, combining imaging, fluorescence and luminescence reading offers efficient analysis for advancing drug screening using 3D cell cultures.
Key learning objectives
- Discover how to generate high-quality spheroids at scale for optimal imaging conditions
- Learn how to perform multiplex analysis of spheroids
- Understand how to simplify and automate spheroid experiments addressing reproducibility, loss of tissue, and analysis challenges
Who should attend?
- Academic scientists interested in the techniques and applications of 3D cell models for research, engineering, and drug development.
- Industry scientists interested in identifying promising platforms for drug discovery and targets for novel therapies using 3D cell models.
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcome summary, for continuing education purposes.
Spark Cyto is for Research Use Only. Not for use in diagnostic procedures.
This webinar was produced in association with SUN bioscience.
Speakers
