Separating polar analyte mixtures: Benefits of a new zwitterionic HILIC chemistry

Hydrophilic interaction chromatography (HILIC) is one of the most effective approaches for separating polar analyte mixtures. Of the stationary phases used for HILIC, zwitterionic chemistries have been among the most popular due to their good retention for a wide range of polar compounds. Waters has developed a new zwitterionic HILIC stationary phase based on bridged-ethylene hybrid (BEH) particles that address needs for improved pH stability, while providing an alternative selectivity to existing HILIC chemistries.

In this SelectScience webinar, we explore the features of this new technology, as well as how using column hardware with inert surfaces can mitigate unwanted interactions between polar analytes that are known to interact with metal surfaces. Applications that demonstrate columns separating several important classes of polar analytes will also be shown.

Key learning objectives

• Explore improvements in zwitterionic HILIC batch-to-batch reproducibility
• Understand the differences in zwitterionic HILIC column selectivity
• Learn the importance of high pH stability when using alkaline mobile phases
• Realize the benefits of mitigating unwanted analyte/hardware surface interactions

Who should attend?

Small molecule method development scientists whose workflows include pharmaceutical, food and environmental, and metabolomics.

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.