Size exclusion native mass spectrometry for the characterization of monoclonal antibodies

Size-exclusion chromatography (SEC) is a well-established method for routine aggregate analysis of monoclonal antibodies (mAbs) and other related biotherapeutics. However, SEC can be combined with high-resolution mass spectrometry (HRMS) to obtain even more detailed characterization data for mAbs. So-called native SEC-MS techniques determine mAb higher order structure, the relative abundance of glycoforms, and fragmentation.

In this presentation, Lorne Nelson, Biopharma Senior Scientist at Phenomenex, will explore how non-denaturing SEC Native MS can be leveraged to streamline mAb characterization. Column selection, including pore volume and column dimensions to ensure monomer and aggregate resolution, will be discussed. The webinar will also cover how to optimize volatile mobile phase composition for improvements in both MS sensitivity and chromatographic performance. Finally, the webinar will detail methods for digestion of mAbs using site-specific proteases to obtain further insight on post-translational modifications.

Attend this webinar to understand:

• How SEC Native MS might be used to provide orthogonal data to other analytical methods
• The critical role of column selection in SEC Native MS
• How to improve MS method sensitivity for volatile mobile phases used in SEC Native MS