Standardization of metagenomics-based microbial profiling

In the past decade, research into human microbiome-based therapeutics and diagnostics has accelerated the widespread availability of next-generation DNA sequencing technologies that comprehensively profile complex microbiota through metagenomic approaches. However, measurements generated by different laboratories often display large variability due to protocol dependent biases introduced at each step in the workflow.

Dr. Dieter Tourlousse, National Institute of Advanced Industrial Science and Technology, and collaborators from the Japan Microbiome Consortium, have established standard operating procedures (SOPs) for metagenomics-based microbiome profiling of human fecal samples. Join this webinar to hear Dr. Tourlousse discuss the steps that were taken to set the SOPs. He will also share how he expects the widespread uptake of the SOPs, which include the QIAseq FX DNA Library Kit for library construction and best-practice guidelines, to increase the accuracy of NGS-based metagenome profiling results across the microbiome field.

Key learning objectives

• Understand the validation and standardization of DNA extraction
• Gain insight into the library construction methods for metagenomics-based human fecal microbiome measurements
• Explore how to compare and assess the reproducibility and transferability of validated protocols in an industry-based interlaboratory study

Who should attend?

Scientists interested in conducting microbiome research

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.