Streamlined DNA Library Preparation Solutions and Guidelines for Robust Quality Control of PCR-free WGS Libraries

Exploring Key Topics in Sample Quality Control for NGS Workflows

Next-generation sequencing (NGS) technologies enable deeper insights into different areas of research. In this latest webinar series, researchers from the European Molecular Biology Laboratory (EMBL), Quantabio LLC,, and Agilent Technologies discuss the importance of input sample quality control in whole genome sequencing (WGS), panel-based targeted sequencing and long-read sequencing workflows in order to maximize sequencing output.

Streamlined DNA Library Preparation Solutions and Guidelines for Robust Quality Control of PCR-free WGS Libraries

Whole genome sequencing (WGS) and panel-based targeted sequencing provide vital genetic information for various applications. The quality of sequencing data hinges on the input library, traditionally prepared using PCR amplification. PCR-free WGS library preparation has become a critical alternative, eliminating amplification-induced issues and preserving the genome's true representation. This is especially important for accurate mutational analysis and disease diagnostics, enhancing the detection of structural variants and GC-rich regions. Quality control (QC) of PCR-free libraries presents a challenge when using electrophoresis. This typically results in inaccurate library size estimations due to the structural features of Y-shaped adapters commonly used during library preparation. In this webinar, we describe a method to accurately determine the size of PCR-free libraries by adding a short PCR amplification step prior to automated DNA fragment analysis.

Key learning objectives:

• Understand why PCR-free WGS library preparation is essential for improving the reliability and efficiency of genetic analyses across various fields
• Discover how sparQ DNA Frag and DNA Library Prep Kits can be utilized to generate PCR-free libraries for DNA library preparation
• Learn how to modify PCR-free libraries for proper QC utilizing the Agilent TapeStation system

Who should attend:

• NGS and genomics researchers
• Molecular biologists
• Cancer researchers
• Sequencing lab managers and technicians

Certificate of attendance

• All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.