Streamlined DNA Library Preparation Solutions and Guidelines for Robust Quality Control of PCR-free WGS Libraries
Exploring Key Topics in Sample Quality Control for NGS Workflows
Next-generation sequencing (NGS) technologies enable deeper insights into different areas of research. In this latest webinar series, researchers from the European Molecular Biology Laboratory (EMBL), Quantabio LLC,, and Agilent Technologies discuss the importance of input sample quality control in whole genome sequencing (WGS), panel-based targeted sequencing and long-read sequencing workflows in order to maximize sequencing output.
Webinar 1 - Sample Integrity Assessment for Long-read Sequencing Technologies, Is It Essential?
Next generation sequencing (NGS) has evolved rapidly in the past decade creating opportunities for new technologies to emerge, ultimately making this technology more robust. Among these, particularly long-read sequencing has experienced an increase of popularity in the research community due to improvements in the overall stability and the quality of generated data.
However, the value of long-read sequencing data is critically dependent on the quality of the input material used for a library preparation. Therefore, reliable and accurate quality control (QC) is essential for the success of these sequencing technologies. At EMBL, the Agilent Femto Pulse system is a fundamental part of the daily routines for processing LRS samples. It allows researchers to verify the integrity and fragment size distribution of nucleic acid samples.
This webinar will highlight the importance of Femto Pulse in GeneCore workflows and present data that emphasize the significance of sample quality control for success of EMBL’s undertakings.
Key learning objectives:
- How stringent QC of incoming samples helps identify and prevent potential problems that could negatively impact their performance in library preparation and sequencing
- How ensuring the accurate size and quantity of the finished library is essential for maximizing sequencing output.
- How the Agilent Femto Pulse system is used in GeneCore workflows.
Dr. Leslie Friedmann
Product Manager for Parallel CE instruments
Agilent Technologies, Germany
Mireia Osuna Lopez, BSc.
Research Technician
European Molecular Biology
Laboratory (EMBL), Germany
Webinar 2 – Streamlined DNA Library Preparation Solutions and Guidelines for Robust Quality Control of PCR-free WGS Libraries
Whole genome sequencing (WGS) and panel-based targeted sequencing provide vital genetic information for various applications. The quality of sequencing data hinges on the input library, traditionally prepared using PCR amplification. PCR-free WGS library preparation has become a critical alternative, eliminating amplification-induced issues and preserving the genome's true representation. This is especially important for accurate mutational analysis and disease diagnostics, enhancing the detection of structural variants and GC-rich regions. Quality control (QC) of PCR-free libraries presents a challenge when using electrophoresis. This typically results in inaccurate library size estimations due to the structural features of Y-shaped adapters commonly used during library preparation. In this webinar, we describe a method to accurately determine the size of PCR-free libraries by adding a short PCR amplification step prior to automated DNA fragment analysis.
Key learning objectives:
- Understand why PCR-free WGS library preparation is essential for improving the reliability and efficiency of genetic analyses across various fields
- Discover how sparQ DNA Frag and DNA Library Prep Kits can be utilized to generate PCR-free libraries for DNA library preparation
- Learn how to modify PCR-free libraries for proper QC utilizing the Agilent TapeStation system
Dr. Daniel Teutsch
Sr. Field Application Specialist
Quantabio LLC, Germany
Dr. Patrick Schindele
Product Manager
Agilent Technologies, Germany
Who should attend:
- NGS and genomics researchers
- Molecular biologists
- Cancer researchers
- Sequencing lab managers and technicians
Certificate of attendance - All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
Speakers
Moderator
