Two-dimensional liquid chromatography (2D-LC): A tutorial focusing on best practice

In recent years, 2D-LC has evolved from a niche technology to one that is in demand, especially when it comes to the analysis of complex samples, or hidden or co-eluting compounds. Whether you are starting out with 2D-LC or want to refresh your knowledge, this two-part webinar series will give you a comprehensive introduction to the technique, including best practices.

Session 1: Key concepts of 2D-LC

Wednesday, September 21, 2022 at 16:00 BST / 17:00 CET / 11:00 EST / 08:00 PST

Learn about the four modes of 2D-LC: heart-cutting, multiple heart-cutting, selective and full comprehensive, and find out how they relate conceptually. Plus, get advice on how to choose complementary separations for the first and second dimension (RP, IEX, HILIC, etc.), how to optimize cycle time, and how to maximize second dimension sensitivity.

Session 2: Solutions and best practices for 2D-LC

Wednesday, October 5, 2022 at 16:00 BST / 17:00 CET / 11:00 EST / 08:00 PST

Get an overview of 2D-LC instrumentation for multi-heart cutting and comprehensive 2D-LC. Gain tips on how to deal with discrepancies between mobile phase dimensions and what to consider for column re-equilibration, detection and minimising additional column dispersion. Plus, hear small molecule application examples from different industries and method development case studies. You can also ask questions to the speaker at the end of this part.

Who should attend?
New and experienced 2D-LC users, scientists, and lab managers or technical decision makers in pharma, biopharma, food, energy & chemicals, environmental, material, forensics, and life science research