![Immunocytochemistry/Immunofluorescence: LC3/MAP1LC3A Antibody [NB100-2331] - LC3 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/0196282f2adb49bb283ea442ece1bb30f88a5885-400x300.jpg?w=3840&q=75&fit=clip&auto=format)
LC3/MAP1LC3A Antibody
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NB100-2331
- Host
- Rabbit
- Reactivity
- Dog (Canine), Fish, Human, Mouse
- Applications
- ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Frozen Sections), Immunohistochemistry (Paraffin-Embedded Sections), Immunoprecipitation, Western Blotting, Southern Blotting
![Immunocytochemistry/Immunofluorescence: Mre11 Antibody [NB100-142] - Detection of MRE11 (Green) in Hela cells using NB100-142 at a 1:200 dilution. Nuclei (Blue) are counterstained using Hoechst 33258.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/a7bcb692240e07d7f4e3817e6d5a446b03f2605b-400x145.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: LC3B/MAP1LC3B Antibody [NB600-1384] - Analysis using the HRP conjugate of NB600-1384. Staining of treated U373-MG cells using NB600-1384. The nuclei were stained with DAPI.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/b4e02b336d8da84d1a200ba1ffd3178f50e33657-400x228.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: HIF-1 alpha Antibody [NB100-449] - Hela cells were treated for 15 hrs with 200uM CoCl2, fixed in PFA, and permeabilized in 90% MeOH. 1 X 10^6 cells were stained with 0.125ug anti- HIF-alpha and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- treated, anti-KLH control IgG; Red- untreated, anti-HIF1-alpha; Blue- treated, anti-HIF1-alpha.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/048f83b9ca705f1fe8583ea09f7991a6dc986ae4-370x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: NBS1 Antibody [NB100-143] - ICC staining of Hela cells using NB100-143 anti-NBS1.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/e95973f30f677f1a98129f0dcfc8098056ac883d-400x296.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - HIF-2 alpha antibody was tested at 1:400 in HepG2 cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/0f5a6a881b7701fb18bd78f04edf5e5b3b38887b-400x322.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: SR-BI Antibody [NB400-104] - Analysis using the HRP conjugate of NB400-104. Detection of SR-BI in 25 ug of mouse liver lysate, using NB400-104 (lotJ) at a 1:1,000 dilution, shows distinct band at 82 kDa.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/11bd3f08898cf23fb4f61ce467217bd1ae44bd6f-241x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis using the HRP conjugate of NB100-123. On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution (NB100-123). The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution. Photo courtesy of Dr. Gregg Semenza, Johns Hopkins University.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/7425c2ef2c3e59c2f444a40f1e36809040da7956-265x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: ATF6 Antibody (70B1413.1) [NBP1-40256] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using ATF6 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/0f4c6bcf323151dbd5b0703be1a0a45d4438993a-400x375.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry: ATM Antibody [NB100-104] - Staining of Testis, Seminiferous Tubule.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/4cd23ad0b2f9db9c6fc65315bda086d112093c7b-400x320.jpg?w=3840&q=75&fit=clip&auto=format)