mCherry Antibody (1C51)
Product Details
- Cat. No.
- NBP1-96752
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
![Western Blot: mCherry Antibody (1C51) [NBP1-96752] - WB assay of the crude extract of Hek293 cells transfected with pFin-EF1-mCherry vector (lane +) and an equal amount of protein extract from untransfected Hek293 cells (lane -). NBP1-96752 binds a major band running at about 28kDa corresponding to intact full-length mCherry. The two other bands are clearly processed forms of mCherry as they are not present in non-transfected Hek293 cells.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/65308a977f52d4cbd356ba400bf1566e5a9ea3a5-214x400.jpg?w=1080&q=75&fit=clip&auto=format)
Western Blot: mCherry Antibody (1C51) [NBP1-96752] - WB assay of the crude extract of Hek293 cells transfected with pFin-EF1-mCherry vector (lane +) and an equal amount of protein extract from untransfected Hek293 cells (lane -). NBP1-96752 binds a major band running at about 28kDa corresponding to intact full-length mCherry. The two other bands are clearly processed forms of mCherry as they are not present in non-transfected Hek293 cells.
![Western Blot: mCherry Antibody (1C51) [NBP1-96752] - WB assay of the crude extract of Hek293 cells transfected with pFin-EF1-mCherry vector (lane +) and an equal amount of protein extract from untransfected Hek293 cells (lane -). NBP1-96752 binds a major band running at about 28kDa corresponding to intact full-length mCherry. The two other bands are clearly processed forms of mCherry as they are not present in non-transfected Hek293 cells.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/65308a977f52d4cbd356ba400bf1566e5a9ea3a5-214x400.jpg?w=256&q=75&fit=clip&auto=format)
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Description
mCherry Antibody (1C51)
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Not Species Specific
- Antigen: mCherry
- Isotype: IgG2a
- Source: Protein G purified
Handling
- Quantity: 0.025 ml
- Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
- Buffer: PBS
- Specificity: 4
Applications
- Immunocytochemistry (ICC)
- Western Blotting (WB)
Good results.
Research use (western blot, co-IP, and ICC)
The anti-mCherry antibody performed well in co-IP and western blot assays but is not good for ICC.
Review Date: 20 Feb 2021 | Novus Biologicals, LLC
![Western Blot: mCherry Antibody (1C51) [NBP1-96752] - WB assay of the crude extract of Hek293 cells transfected with pFin-EF1-mCherry vector (lane +) and an equal amount of protein extract from untransfected Hek293 cells (lane -). NBP1-96752 binds a major band running at about 28kDa corresponding to intact full-length mCherry. The two other bands are clearly processed forms of mCherry as they are not present in non-transfected Hek293 cells.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/65308a977f52d4cbd356ba400bf1566e5a9ea3a5-214x400.jpg?w=3840&q=75&fit=clip&auto=format)