12 nm Colloidal Gold-AffiniPure Donkey Anti-Sheep IgG (H+L) (min X Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot) (EM Grade)
Product Details
- Cat. No.
- 713-205-147
- Type
- Secondary Antibody
- Clonality
- Polyclonal
- Host
- Donkey
Description
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule sheep IgG. It also reacts with the light chains of other sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with chicken, guinea pig, syrian hamster, horse, human, mouse, rabbit and rat serum proteins, but it may cross-react with immunoglobulins from other species.
Biological Information
- Clonality: Polyclonal
- Host: Donkey
- Reactivity: Sheep (Ovine)
Handling
- Quantity: 0.3 ml
- Storage: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Prepare working dilution fresh each day. Expiration date: six months from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
- Buffer: 0.01M Sodium Borate - Sodium Phosphate, 0.15M NaCl, pH 8.5