Product & ReviewsAntibodies

CF®350 Goat Anti-Mouse IgG2a (γ2a), 2mg/mL

Product Details

Cat. No.
#20255
Type
Secondary Antibody
Clonality
Polyclonal
Host
Goat
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Biotium

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Description

Goat anti-mouse IgG2a isotype-specific secondary antibody labeled with CF®350 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. UV-excitable blue fluorescent CF®350 dye has excitation/emission at 347/448 nm. Note: Conjugates of blue fluorescent dyes like CF®350, CF®405S, and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.The conjugate is prepared from affinity-purified antibodies that react with Fc portion of the heavy chain of mouse IgG2a. To minimize cross reactivity, the antibodies are cross-adsorbed against other mouse IgG subclasses (IgG1, IgG2b, IgG3), and human, bovine and rabbit serum proteins.

Biological Information

  • Clonality: Polyclonal
  • Host: Goat
  • Conjugate: CF®350

Handling

  • Quantity: 250uL
  • Storage: Store at -10 to -35 °C|Protect fluorescent conjugates from light
  • Buffer: PBS, 50% glycerol, 2 mg/ml BSA, 0.05% azide

Applications

  • Immunofluorescence (IF)
  • Immunohistochemistry (Formalin-Fixed Sections) (IHC (F))
  • Immunohistochemistry (Frozen Sections) (IHC (Fr))
  • Western Blotting (WB)
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