5-Hydroxymethylcytosine (5-hmC) mouse antibody (mAb) (Clone 59.1)
Product Details
- Cat. No.
- 39999
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
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Description
DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-Hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. This antibody was developed specifically to distinguish 5-hydroxymethylcytosine from 5-methylcytosine as conventional methods (enrichment by antibody or methyl DNA binding protein, enzymatic digestion and bisulfite sequencing) cannot do so. It is possible that 5-hydroxymethylcytosine (5-hmC) represents a pathway to demethylate DNA, as 5-hydroxymethylcytosine is repaired as mismatched DNA and replaced with unmethylated cytosine.
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Not Species Specific, Human, Mouse
- Clone: 59.1
- Isotype: IgG2a
Handling
- Quantity: 100 ug
- Storage: Antibodies in solution can be stored at -20C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Specificity: 5
Applications
- Dot Blot (DB)
- Immunofluorescence (IF)
- Methylated DNA Immunoprecipitation (MeDIP)
Performs well without lot variability.
ELIZA/DB/HTRF
We applied this antibody as we wanted monoclonal for high-throughput screen. This ab was superior with respect to specificity and sensitivity to other mAbs from Millipore and Diagenode. Excellent thing is that it retained reactivity even after conjugation to a chemical dye. Sensitivity is inferior to anti-5hmC pAb from Active Motif.
Review Date: 16 Sept 2016 | Active Motif