5-Hydroxymethylcytosine (5-hmC) rabbit antibody (pAb)
Product Details
- Cat. No.
- 39791
- Type
- Primary Antibody
- Clonality
- Polyclonal
- Host
- Rabbit
The supplier does not provide quotations for this antibody through SelectScience. You can search for similar antibodies in our Antibody Directory.
Description
DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-Hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. This antibody was developed specifically to distinguish 5-hydroxymethylcytosine from 5-methylcytosine as conventional methods (enrichment by antibody or methyl DNA binding protein, enzymatic digestion and bisulfite sequencing) cannot do so. It is possible that 5-hydroxymethylcytosine (5-hmC) represents a pathway to demethylate DNA, as 5-hydroxymethylcytosine is repaired as mismatched DNA and replaced with unmethylated cytosine.
Biological Information
- Clonality: Polyclonal
- Host: Rabbit
- Reactivity: Not Species Specific, Human, Mouse
- Isotype: IgG
Handling
- Quantity: 100 ug
- Storage: Antibodies in solution can be stored at -20C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Specificity: 5
Applications
- Dot Blot (DB)
- Methylated DNA Immunoprecipitation (MeDIP)
Highly specific and robust. Superior to other anti-hmC antibodies.
ELISA, DB, hMeDIP
Sensitivity was superior among 5 anti-5-hmC antibodies tested. Specificity was also good enough and did not see significant signals on unmodified, mC, fC or caC-containing synthetic DNA. Used routinely for DB. Also worked well in hMEDIP, IF and IHC (worked in all applications tested so far).
Review Date: 16 Sept 2016 | Active Motif