6nm Colloidal Gold-AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu,Ms,Rat Sr Prot) (EM Grade)
Product Details
- Cat. No.
- 111-195-144
- Type
- Secondary Antibody
- Clonality
- Polyclonal
- Host
- Goat
Description
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, mouse and rat serum proteins, but it may cross-react with immunoglobulins from other species.
Biological Information
- Clonality: Polyclonal
- Host: Goat
- Reactivity: Rabbit (Lapine)
Handling
- Quantity: 0.5 ml
- Storage: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Prepare working dilution fresh each day. Expiration date: six months from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
- Buffer: 0.01M Sodium Borate - Sodium Phosphate, 0.15M NaCl, pH 8.5
- Specificity: 4
Applications
Electron microscopy immunolabeling
EM grade colloidal Gold conjugates have been very consistent and yielding good results
Review Date: 16 Sept 2016 | Jackson ImmunoResearch Laboratories Inc.