Acetylated Lysine Antibody (1C6)
Product Details
- Cat. No.
- NB100-74339
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
![Chromatin Immunoprecipitation: Acetylated Lysine Antibody (1C6) [NB100-74339] - Analysis of chromatin proteins with acetylated lysines was performed using cross-linked chromatin from 4x10^6 LNCaP cells. Immunoprecipitation was performed using the Magnetic ChIP kit with 10ug of an Acetyl Lysine monoclonal antibody. Quantitative real-time PCR data was obtained using a Thermo PikoReal PCR System, SYBR Green PCR Master Mix, and primers (5’-TACTAGCGGTTTTACGGGCG, 3’-TCGAACAGGAGGAGCAGAGAGCGA) flanking the human GAPDH promoter proximal to the start site of transcription. Quantitation of immunoprecipitated GAPDH promoter sequence is presented as fold enrichment of the acetyl lysine monoclonal antibody versus non-specific IgG.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/fc3ab40bfb4b87c7f2413880b0dd40cd60eb64e8-400x291.jpg?w=1080&q=75&fit=clip&auto=format)
Chromatin Immunoprecipitation: Acetylated Lysine Antibody (1C6) [NB100-74339] - Analysis of chromatin proteins with acetylated lysines was performed using cross-linked chromatin from 4x10^6 LNCaP cells. Immunoprecipitation was performed using the Magnetic ChIP kit with 10ug of an Acetyl Lysine monoclonal antibody. Quantitative real-time PCR data was obtained using a Thermo PikoReal PCR System, SYBR Green PCR Master Mix, and primers (5’-TACTAGCGGTTTTACGGGCG, 3’-TCGAACAGGAGGAGCAGAGAGCGA) flanking the human GAPDH promoter proximal to the start site of transcription. Quantitation of immunoprecipitated GAPDH promoter sequence is presented as fold enrichment of the acetyl lysine monoclonal antibody versus non-specific IgG.
![Chromatin Immunoprecipitation: Acetylated Lysine Antibody (1C6) [NB100-74339] - Analysis of chromatin proteins with acetylated lysines was performed using cross-linked chromatin from 4x10^6 LNCaP cells. Immunoprecipitation was performed using the Magnetic ChIP kit with 10ug of an Acetyl Lysine monoclonal antibody. Quantitative real-time PCR data was obtained using a Thermo PikoReal PCR System, SYBR Green PCR Master Mix, and primers (5’-TACTAGCGGTTTTACGGGCG, 3’-TCGAACAGGAGGAGCAGAGAGCGA) flanking the human GAPDH promoter proximal to the start site of transcription. Quantitation of immunoprecipitated GAPDH promoter sequence is presented as fold enrichment of the acetyl lysine monoclonal antibody versus non-specific IgG.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/fc3ab40bfb4b87c7f2413880b0dd40cd60eb64e8-400x291.jpg?w=256&q=75&fit=clip&auto=format)
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Description
Acetylated Lysine Antibody (1C6)
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Human, Mouse
- Antigen: Acetylated Lysine
- Isotype: IgG
- Source: Protein G purified
Handling
- Quantity: 0.1 mg
- Storage: Store at -20C. Avoid freeze-thaw cycles.
- Buffer: PBS and 1 mg/ml BSA
Applications
- Chromatin Immunoprecipitation (ChIP)
- Dot Blot (DB)
- Immunocytochemistry (ICC)
- Western Blotting (WB)
![Chromatin Immunoprecipitation: Acetylated Lysine Antibody (1C6) [NB100-74339] - Analysis of chromatin proteins with acetylated lysines was performed using cross-linked chromatin from 4x10^6 LNCaP cells. Immunoprecipitation was performed using the Magnetic ChIP kit with 10ug of an Acetyl Lysine monoclonal antibody. Quantitative real-time PCR data was obtained using a Thermo PikoReal PCR System, SYBR Green PCR Master Mix, and primers (5’-TACTAGCGGTTTTACGGGCG, 3’-TCGAACAGGAGGAGCAGAGAGCGA) flanking the human GAPDH promoter proximal to the start site of transcription. Quantitation of immunoprecipitated GAPDH promoter sequence is presented as fold enrichment of the acetyl lysine monoclonal antibody versus non-specific IgG.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/fc3ab40bfb4b87c7f2413880b0dd40cd60eb64e8-400x291.jpg?w=3840&q=75&fit=clip&auto=format)