AffiniPure Goat Anti-Human IgG, Fc? Fragment Specific (min X Bov,Hrs,Ms Sr Prot)
Product Details
- Cat. No.
- 109-005-098
- Type
- Secondary Antibody
- Clonality
- Polyclonal
- Host
- Goat
Description
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species.
Biological Information
- Clonality: Polyclonal
- Host: Goat
- Reactivity: Human
Handling
- Quantity: 1 mg
- Storage: Store at 2-8°C under sterile conditions. Prepare working dilution fresh each day. Expiration date: one year from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
- Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
- Specificity: 5
Applications
ELISA - detecting IgG secreted from human cells
This antibody functions very well as a capture antibody for detecting human IgG secreted from PBMC populations.
Review Date: 27 Oct 2020 | Jackson ImmunoResearch Laboratories Inc.
ELISA , Luminex
It's easy to use, stable at 4 degree. It's the best one after we compare with the other company's product.
Review Date: 16 Sept 2016 | Jackson ImmunoResearch Laboratories Inc.