Product & ReviewsAntibodies
AKT (phospho-Ser473) antibody
Product Details
- Cat. No.
- orb127667
- Type
- Primary Antibody
- Clonality
- Polyclonal
- Host
- Rabbit
Western blot analysis of extracts from K562 cells using CIITA antibody
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Description
Rabbit polyclonal antibody to AKT (phospho-Ser473)
Biological Information
- Clonality: Polyclonal
- Host: Rabbit
- Reactivity: Human, Mammalian, Mouse
- Isotype: IgG
- Gene: 2641
Handling
- Quantity: 100 µl, 200 µl, 50 µl
- Storage: Store at -20°C. Avoid freeze / thaw cycles
- Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
- Specificity: 5
Applications
- ELISA (ELISA)
- Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P))
- Western Blotting (WB)
Highly specific antibody with good sensitivity
Application Area:
Western, IP and IF
For western, I used 1:1000 dilution and prepared the antibody in 3% BSA. It worked efficiently. A single band at the expected size was obtained. For IF, I used 1:50 dilution in % BSA solution prepared in 1X PBS. For IP, I used 2ug for 500 ug of whole cell lysate of the MCF-7 cell line. It's a very good antibody and provides reproducible results.
Review Date: 30 Sept 2022 | Biorbyt
