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F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, syrian hamster, horse, human, mouse, rabbit and sheep serum proteins, but it may cross-react with immunoglobulins from other species.
Performs exceptionally well, giving beautiful fluorescence staining.
Immunofluorescence
The antibody arrived within 2 days after ordering. We have used this secondary antibody to detect a rat anti-mouse CD41 antibody and it works wonders! Low background staining and a very bright signal in the green fluorescence channel.
Review Date: 12 Dec 2019 | Jackson ImmunoResearch Laboratories Inc.