Product & ReviewsAntibodies
Alpha Tubulin Monoclonal antibody
Product Details
- Cat. No.
- 66031-1-Ig
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
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Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Dog (Canine), Human, Mouse, Rat
- Isotype: IgG2b
Handling
- Quantity: 150 μL
- Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
- Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
- Specificity: 5
Applications
- ELISA (ELISA)
- Flow Cytometry (FC)
- Immunofluorescence (IF)
- Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P))
- Immunoprecipitation (IP)
- Western Blotting (WB)
Beautiful staining.
Application Area:
Molecular biology, cell biology
Alpha tubulin works great for us in WB and immunofluorescence. For WB we need to use at 1:1000 which is not great because the antibodies go fast, but the resulting band is sharp and clear. We use it for cell lines 250,000 cels per lane, 1:30h RT or 4 degrees overnight, and tissue extracts (30ug/lane). For immunofluorescence, we see beautiful strong and specific staining at 1:200. Recommended!
Review Date: 20 Feb 2021 | Proteintech Group
