Anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, AFDyeTM 647 Conjugate
Product Details
- Cat. No.
- 61058-647
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
WB with anti-AAV VP1/VP2/VP3 AFDyeTM 647 antibody (Cat. No. 61058-647, 0.2 µg/ml) using capsids of different AAV serotypes as sample.
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Description
The B1 antibody reacts with free VP1, VP2 and VP3 of adeno-associated virus (AAV) and at a reduced degree with assembled viral particles. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus et al., 2000) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection.The AFDye™ 647 is a bright, far-red-fluorescent dye ideally suited for the 633 nm or 647 nm laser lines (excitation/emission 648/671 nm). It is structurally similar to Alexa Fluor® 647, and can be used with a common Cy5 filter set. A significant advantage to using long wavelength dyes such as Cy5 or AFDye™ 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: AAV1 , AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAVDJ
- Antigen: AAV2 capsids
- Clone: B1
- Conjugate: AFDyeTM 647
- Isotype: IgG1
Handling
- Quantity: 50 µg (100 µl)
- Storage: up to 1 month: 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles; store in the dark.
Applications
- Dot Blot (DB)
- Western Blotting (WB)
