anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, lyophilized, purified
Product Details
- Cat. No.
- 61058
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
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Description
B1 reacts with free VP1, VP2 and VP3 of adeno-associated virus (AAV) and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus et al. 2000) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection. Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Adeno-Associated Virus
- Antigen: AAV2 capsids
- Clone: B1
- Conjugate: unconjugated
- Isotype: IgG1
Handling
- Quantity: 50 µg
- Storage: lyophilized antibody: 2-8°C; reconstituted antibody: short term at 2-8°C; for long term storage we recommend to add 0.5% BSA prior to storing in aliquots at -20°C; avoid freeze/thaw cycles
Applications
- Affinity Chromatography (AC)
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blotting (WB)